Virus Detection by On-chip Hydroxyapatite Chromatography

We have succeeded in isolating virus from the particle laden solution like body fluid. Because many of virus infectious diseases occur in the developing region, compact and low-cost device for virus detection from the patients’ body fluid is required. We proposed a microfluidic chip for virus isolation by hydroxyapatite chromatography. This microfluidic chip utilized a column and a pair of switching valves. In the upstream and downstream part of the column, cylindrical micropillars are placed to hold the hydroxyapatite particles in the column. Chromatography was carried out in this column and the viruses were successfully isolated by buffer displacement.